Protein A Affinity Chromatography Medium UniMab 50L Recombinant->chemical

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Protein A Affinity Chromatography Medium UniMab 50L Recombinant

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Chemicals->Others->chemical

Protein A Affinity Chromatography Medium UniMab 50L Recombinant

Product Specification

Model No:	UniMab 50L Recombinant

Country	CN
Payment Term	L/C,T/T

Product Detail

<h2 align="center" style="text-align:center;">
Protein A Affinity Chromatography Medium
</h2>

 


     Uni®Mab is a high performance Protein A affinity
chromatography medium, introduced by Nanomicrotechwithits patented technology
developed in-house. This medium is based on the monodisperse porous poly
(methyl methacrylate) (PMMA) microspheres as the matrix, and a genetically
modified protein A as the ligand, which ensures its high affinity binding of
monoclonal antibodies and Fc fragment containing recombinant proteins
(macromolecules). Uni®Mab has come with high mechanical strength and good pH
stability. Even at a high flow rate, it still maintained a high dynamic binding
capacity, meeting the demands from laboratory preparationto industrial
production.


 <img src="http://i.bosscdn.com/product/b0/f5/7b/dfd783adfb4523846c6b82f364.png" width="345" height="205" title="Affinity Chromatography Procedure" alt="Affinity Chromatography Procedure" /> 


Characteristics and Advantages 


<img src="http://i.bosscdn.com/product/0a/d8/56/a591219ee01640737618ef9855.png" width="454" height="154" title="Chromatography Separation and Purification" alt="Chromatography Separation and Purification" /> 


Highly uniform
particles 
    Uni®Mab affinity
chromatography medium has highly uniform particle size distribution and
precisely controlled porestructure, as shown in Fig. 1.


<img src="http://i.bosscdn.com/product/17/08/89/7b0b98f764ad48c01f9cd57bf2.png" width="425" height="182" title="Protein A Sepharose" alt="Protein A Sepharose" /> 


Figure 1. SEM
diagram of Uni®Mab and a leading brand of protein A medium


High flow rate 
    Uni®Mab affinity
chromatography medium was made from polymethyl methacrylate (PMMA) microspheres
(as matrix); and it would withstand the pressure of 0.5 MPa. When
compared to an affinity medium with convention alagarose matrix of the
chromatography media, it would make the separation process much faster, thus
reducing the time purification, for hundreds of liters of large scale
chromatography preparative column.(Experimental:Column:4.6 mm × 50 mm   Mobile phase:50 mM PBS,0.5 M NaCl)


<img src="http://i.bosscdn.com/product/02/f9/75/ab705e71db11dd09170359a242.png" width="352" height="275" title="Protein Purification Methods" alt="Protein Purification Methods" /> 
Figure 2. Comparison of Uni®Mab and a
conventional agarose type protein A medium, their pressure vs. linear flow
rate.


Excellent loading
capacity 
At a high flow rate, Uni®Mab showed a
significantly better dynamic binding capacity (DBC) than that of the
conventional agarose type protein A medium.  The same observation was also
valid, even when the residence time was increase to 4 - 6 minute.(Experimental:Column:7 mm × 25 mm   Sample:Human IgG,2 mg/mL    Equilibration
buffer:20 mM PBS,pH 7.0 ,0.15 M NaCl    Elution:0.1 M Glycine ,pH 3.0)


<img src="http://i.bosscdn.com/product/2d/67/85/694545ee53af6965b13cc66792.png" width="290" height="174" title="Protein A Chromatography" alt="Protein A Chromatography" /> 
Figure 3. Comparison of Uni®Mab with a
leading brand of Protein A medium, for their dynamic binding capacity


Outstanding pH
stability 
Uni®Mab affinity chromatography medium could be
applied in a pH range from 3 - 12, and it could be cleaned with 0.1 - 0.5 M
NaOH. In a life-cycle test, Uni®Mab medium
remained greater than 90% of the original dynamic binding capacity (DBC), after
100 CIP cycles with 0.5 M NaOH as CIP reagent.  (CIP procedure:Medium:Uni®Mab Column:7 mm × 25 mm   Sample:recombinant antibody(chimera)    DBC
calculation:10% breakthrough   Equilibration
buffer:20 mM PBS,150 mM NaCl, pH 7.2,3 CV(column volume)
  Elution:20 mM Citrate,pH 3.0  CIP:0.5 M NaOH,15 CV  Re-equilibration:10 CV)


<img src="http://i.bosscdn.com/product/1e/b5/2d/09a1d6caa0173511147df31800.png" width="351" height="241" title="Protein Purification Techniques" alt="Protein Purification Techniques" /> 
Figure 4. Uni®Mab pH stability in life cycle tests


Application 
Uni®Mab was secessfully applied in purification of
hIgG from human serum. (Experimental:Column:7 mm× 25 mm  Sample:human serum,5 mL  Equilibration Buffer:20mM PBS,pH 7.0


0.15 M NaCl
  Elution:0.1 M Glycine ,pH 3.0  Residence time:4 min )


<img src="http://i.bosscdn.com/product/f4/0a/00/de973b48a12d7130dfe6c56cd2.png" width="424" height="289" title="Protein A Affinity Chromatography" alt="Protein A Affinity Chromatography" /> 
Figure 5.Uni®Mab used in hIgG purification from human serum


  Technical
information 


<img src="http://i.bosscdn.com/product/e8/3f/d2/c964c045448a502e99bdf6b67e.png" width="451" height="256" title="Purification Of Proteins" alt="Purification Of Proteins" /> 


Pre-packed column cartridge 


<img src="http://i.bosscdn.com/product/33/b1/26/d345f49bd4a2b4488680e256d0.png" width="492" height="157" title="Protein A Purification" alt="Protein A Purification" /> 


About Nanomicrotech 
   
  We are proud to be the sole producer (and provider) of monodisperse,
super pure silica gel chromatographic media in the world; we also provide
monodisperse polymer chromatographic media (these products will match the
performance of similar GE products). Nanomicrotech Co. is one of a few
companies in the world, with a capacity of commercial-scale production for both
polymer chromatographic media and super pure silica gel media. Currently, the
company currently offers most kinds of purification media, covering the
positive phase, reverse phase, ion exchange, hydrophobic and affinity
chromatography.


 

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</div>nanomicro-technology.com

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